mdh.sePublications
Change search
Refine search result
1 - 2 of 2
CiteExportLink to result list
Permanent link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf
Rows per page
  • 5
  • 10
  • 20
  • 50
  • 100
  • 250
Sort
  • Standard (Relevance)
  • Author A-Ö
  • Author Ö-A
  • Title A-Ö
  • Title Ö-A
  • Publication type A-Ö
  • Publication type Ö-A
  • Issued (Oldest first)
  • Issued (Newest first)
  • Created (Oldest first)
  • Created (Newest first)
  • Last updated (Oldest first)
  • Last updated (Newest first)
  • Disputation date (earliest first)
  • Disputation date (latest first)
  • Standard (Relevance)
  • Author A-Ö
  • Author Ö-A
  • Title A-Ö
  • Title Ö-A
  • Publication type A-Ö
  • Publication type Ö-A
  • Issued (Oldest first)
  • Issued (Newest first)
  • Created (Oldest first)
  • Created (Newest first)
  • Last updated (Oldest first)
  • Last updated (Newest first)
  • Disputation date (earliest first)
  • Disputation date (latest first)
Select
The maximal number of hits you can export is 250. When you want to export more records please use the Create feeds function.
  • 1.
    de Maré, L
    et al.
    Lund Institute of Technology, Sweden.
    Velut, S
    Lund Institute of Technology, Sweden.
    Ledung, Erika
    Mälardalen University, Department of Biology and Chemical Engineering.
    Cimander, C
    Novozymes Biopharma AB, Lund, Sweden .
    Norrman, Bo
    Mälardalen University, Department of Biology and Chemical Engineering.
    Karlsson, E
    Lund Institute of Technology, Lund, Sweden.
    Holst, O
    Lund Institute of Technology, Lund, Sweden.
    Hagander, P
    Lund Institute of Technology, Sweden.
    A cultivation technique for E. coli fed-batch cultivations operating close to the maximum oxygen transfer capacity of the reactor2005In: Biotechnology Letters, ISSN 0141-5492, Vol. 27, no 14, p. 983-990Article in journal (Refereed)
    Abstract [en]

    A cultivation strategy combining the advantages of temperature-limited fed-batch and probing feeding control is presented. The technique was evaluated in fed-batch cultivations with E. coli BL21(DE3) producing xylanase in a 3 liter bioreactor. A 20% increase in cell mass was achieved and the usual decrease in specific enzyme activity normally observed during the late production phase was diminished with the new technique. The method was further tested by growing E. coli W3110 in a larger bioreactor (50 l). It is a suitable cultivation technique when the O2 transfer capacity of the reactor is reached and it is desired to continue to produce the recombinant protein.

  • 2.
    Sundström, Heléne
    et al.
    Royal Institute of Technology, Sweden.
    Wållberg, Fredrik
    Royal Institute of Technology, Sweden.
    Ledung, Erika
    Mälardalen University, Department of Biology and Chemical Engineering.
    Norrman, Bo
    Mälardalen University, Department of Biology and Chemical Engineering.
    Hewitt, Christopher J
    University of Birmingham, UK.
    Enfors, Sven-Olof
    Royal Institute of Technology, Sweden.
    Segregation to non-dividing cells in recombinant Escherichia coli fed-batch fermentation processes2004In: Biotechnology Letters, ISSN 0141-5492, Vol. 26, no 19, p. 1533-1539Article in journal (Refereed)
    Abstract [en]

    In Escherichia coli fermentation processes, a drastic drop in viable cell count as measured by the number of colony forming units per ml (c.f.u. ml(-1)) is often observed. This phenomenon was investigated in a process for the production of the recombinant fusion protein, promegapoietin (PMP). After induction, the number of c.f.u. ml(-1) dropped to approximately 10% of its maximum though the biomass concentration continued to increase. Flow cytometric analysis of viability and intracellular concentration of PMP showed that almost all cells were alive and contributed to the production. Thus, the drop in the number of c.f.u. ml(-1) probably reflects a loss of cell division capability rather than cell death.

1 - 2 of 2
CiteExportLink to result list
Permanent link
Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
  • text
  • asciidoc
  • rtf