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  • 1.
    Muter, Olga
    et al.
    University of Latvia.
    Nehrenheim, Emma
    Mälardalen University, School of Sustainable Development of Society and Technology.
    Odlare, Monica
    Mälardalen University, School of Sustainable Development of Society and Technology.
    Rodriguez, Adrian
    Mälardalen University, School of Sustainable Development of Society and Technology.
    Demilitarization industry sludge: assessment of toxicity and biodegradation potential2009Conference paper (Refereed)
  • 2.
    Nehrenheim, Emma
    et al.
    Mälardalen University, School of Business, Society and Engineering, Future Energy Center.
    Muter, Olga
    Latvia University.
    Odlare, Monica
    Mälardalen University, School of Business, Society and Engineering, Future Energy Center.
    Rodriguez, Adrian
    Mälardalen University, School of Business, Society and Engineering, Future Energy Center. Institute for Catalan Water Research.
    Cepurnieks, Guntis
    National Diagnostic Centre, Latvia.
    Bartkevics, Vadims
    National Diagnostic Centre, Latvia.
    Toxicity assessment and biodegradation potential of water-soluble sludge containing 2,4,6-trinitrotoluen2013In: Water Science & Technology, ISSN 0273-1223, Vol. 68, no 8, p. 1707-1714Article in journal (Refereed)
    Abstract [en]

    he water-soluble phase of trinitrotoluene-containing sludge (SLP) was characterized with regard to trinitrotoluene (TNT) concentration, ecotoxicity, and a model biodegradation experiment as evaluation criteria for further development of appropriate treatment technologies. SLP contained 67.8 mg TNT/l. The results of germination and root-elongation tests indicated that SLP had a species-specific phytotoxic effect. The results of a 21 day degradation experiment demonstrated TNT conversion to 4-amino-2,6-DNT and 2-amino-4,6-DNT, with a simultaneous reduction in the total concentration of nitroaromatics. Addition of inoculum stimulated the TNT degradation process. The presence of the sludge solid phase inhibited microbial activity. Measurement of microbial enzyme activity was used to assess changes in the microbial community during the biodegradation process.

  • 3.
    Nehrenheim, Emma
    et al.
    Mälardalen University, School of Sustainable Development of Society and Technology.
    Odlare, Monica
    Mälardalen University, School of Sustainable Development of Society and Technology.
    Allard, Bert
    Örebro Universitet.
    Rodriguez, Adrian
    Mälardalen University, School of Sustainable Development of Society and Technology.
    Treatment of metal contaminated water by using pine bark – a multivariate approach2009Conference paper (Refereed)
  • 4.
    Nehrenheim, Emma
    et al.
    Mälardalen University, School of Sustainable Development of Society and Technology.
    Rodriguez, Adrian
    Mälardalen University, School of Sustainable Development of Society and Technology.
    Odlare, Monica
    Mälardalen University, School of Sustainable Development of Society and Technology.
    Johansson Westholm, Lena
    Mälardalen University, School of Sustainable Development of Society and Technology.
    Wastewater phosphorous removal by blast furnace slag: Laboratory and field investigations in Sweden2009Conference paper (Refereed)
  • 5.
    Odlare, Monica
    et al.
    Mälardalen University, School of Sustainable Development of Society and Technology.
    Nehrenheim, Emma
    Mälardalen University, School of Sustainable Development of Society and Technology.
    Rodriguez, Adrian
    Mälardalen University, School of Sustainable Development of Society and Technology.
    Microbiological degradation of explosives in bioreactor – experiences from Nammo Vingåkersverken.2008Conference paper (Refereed)
  • 6.
    Rodriguez, Adrian
    et al.
    Mälardalen University, School of Sustainable Development of Society and Technology.
    Påhlson, Carl
    Mälardalen University, School of Sustainable Development of Society and Technology.
    Odlare, Monica
    Mälardalen University, School of Sustainable Development of Society and Technology.
    Dahlquist, Erik
    Mälardalen University, School of Sustainable Development of Society and Technology.
    Färm, Carina
    Mälardalen University, School of Sustainable Development of Society and Technology.
    Fluorescence in situ hybridization (FISH) for quantification of species of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in wastewater treatment activated sludge.2009Conference paper (Refereed)
  • 7.
    Rodriguez Caballero, Adrian
    Mälardalen University, School of Sustainable Development of Society and Technology.
    STUDY OF BACTERIAL COMMUNITIES: – A WASTEWATER TREATMENT PERSPECTIVE2011Licentiate thesis, comprehensive summary (Other academic)
    Abstract [en]

    In this thesis, the application of molecular microbiology methods to understand wastewater treatment bio-reactions is described. Two different wastewater treatment systems were chosen for the experimental work. Firstly; the activated sludge processes at two different facilities in Sweden (Västerås and Eskilstuna) were investigated and compared in a context where low temperatures can affect the efficiency of the nitrogen removal performance in terms of nitrification. Initially, fluorescence in situ hybridization (FISH) was utilised in order to quantify some of the species involved in ammonia and nitrite oxidation at Västerås, providing information on how the different communities react to decreasing temperatures. Then, the polymerase chain reaction (PCR), cloning-sequencing method was employed in order to study the composition of the ammonia oxidizing bacteria (AOB) community at the same two wastewater treatment plants (WWTPs). Secondly; the potential use of constructed wetlands for the treatment of winery wastewater was studied. High ethanol concentration artificial wastewater with and without inorganic nutrients (nitrates and phosphates) was fed in a set of pilot-scale constructed wetlands. Pollutant removal performance and enzyme activity tests were carried out. Additionally, the bacterial community structure was investigated by means of denaturing gradient gel electrophoresis (DGGE).

    In the first set of studies it was shown that the AOB population which plays a major role in nitrifying reactors presented a seasonal shift and a higher diversity at Västerås during winter time, while the nitrification performance maintained stable levels and the ammonia removal efficiency increased. Thus, the higher ammonia removal efficiency at Västerås could be related to the diversity of the AOB population composition. Lastly, when constructed wetlands were in focus, the differential effects of ethanol and nutrients over the chemical oxygen demand (COD) removal performance were proven. In fact, the addition of nutrients on one of the experimental wetlands increased the COD (ethanol) removal and supported the maintenance of a bacterial population similar to the control wetland (no ethanol added). In conclusion, both studies proved a strong relationship between process performance (pollution removal) and the dynamics of the bacterial communities involved.

  • 8.
    Rodriguez Caballero, Adrian
    Mälardalen University, School of Sustainable Development of Society and Technology.
    TREATMENT OF HIGH ETHANOL CONCENTRATION WASTEWATER BY CONSTRUCTED WETLANDS: ENHANCED COD REMOVAL AND BACTERIAL COMMUNITY DYNAMICS2011Conference paper (Other academic)
    Abstract [en]

    Winery wastewater is characterized by its high chemical oxygen demand (COD), seasonal occurrence and variable composition, including periodic high ethanol concentrations. In addition, winery wastewater may contain insufficient inorganic nutrients for optimal biodegradation of organic constituents. Two pilot-scale constructed wetlands (CWs) were used to treat artificial wastewater: the first was amended with ethanol and the second with ethanol, inorganic nitrogen (N) and phosphorus (P). A number of biochemical parameters involved in the degradation of pollutants through CW systems were monitored, including effluent chemistry and bacterial community structures. The nutrient supplemented CW showed efficient COD, N and P removal. Comparison of the COD removal efficiencies of the two CWs showed that N and P addition enhanced COD removal efficiency by up to 16%. Molecular fingerprinting of CW sediment samples using denaturing gradient gel electrophoresis (DGGE) showed that amendment with high concentrations of ethanol destabilized the microbial community structure, but that nutrient supplementation countered this effect.

  • 9.
    Rodriguez Caballero, Adrian
    et al.
    Mälardalen University, School of Sustainable Development of Society and Technology.
    Hallin, S
    Swedish Univ Agr Sci.
    Påhlson, Carl
    Uppsala Univ.
    Odlare, Monica
    Mälardalen University, School of Business, Society and Engineering.
    Dahlquist, Erik
    Mälardalen University, School of Business, Society and Engineering.
    Ammonia oxidizing bacterial community composition relates to process performance in wastewater treatment plants under low temperature conditions2012In: Water Science and Technology, ISSN 0273-1223, E-ISSN 1996-9732, Vol. 65, no 2, p. 197-204Article in journal (Other academic)
    Abstract [en]

    Nitrification can be difficult to maintain at wastewater treatment plants (WWTPs) during cold periods resulting in disrupted nitrogen removal. The aim of this study was to relate nitrification process performance to abundance and composition of the ammonia oxidizer communities in two closely located municipal WWTPs in Sweden during an eight month period covering seasonal changes and low temperature conditions. Both facilities showed lower NH4+-N removal efficiency and nitrification rates as temperature decreased. However, one of the plants had a more stable nitrification rate and higher ammonia removal efficiency throughout the entire period. The differences in performance was related to a shift in the composition of the bacterial ammonia oxidizing community from a Nitrosomonas oligotropha-dominated community to a mixed community including also Nitrosomonas ureae-like ammonia oxidizers. This was likely a response to differences in NH4+-N and organic loading.

  • 10.
    Rodriguez Caballero, Adrian
    et al.
    Mälardalen University, School of Sustainable Development of Society and Technology.
    Odlare, Monica
    Mälardalen University, School of Sustainable Development of Society and Technology.
    Ramond., J-B
    Cape Peninsula University of Technology .
    Welz, P.J
    Cape Peninsula University of Technology .
    Cowan, D.A.,
    Cape Peninsula University of Technology .
    Treatment of high ethanol concentration wastewater by constructed wetlands: enhanced COD removal and bacterial community dynamics.2010In: Microbes in Wastewater & Waste Treatment, Bioremediation and Energy Production, 2010Conference paper (Refereed)
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