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Bacteria counting with impedance spectroscopy in a micro probe station
Uppsala University, Sweden.
Uppsala University, Sweden.
Mälardalen University.
Uppsala University, Sweden.
2006 (English)In: Journal of Physical Chemistry B, ISSN 1520-6106, E-ISSN 1520-5207, Vol. 110, no 20, 10165-10169 p.Article in journal (Refereed) Published
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Abstract [en]

A method to quantify the density of viable biological cells in suspensions is presented. The method is implemented by low-frequency impedance spectroscopy and based on the finding that immobilized ions are released to move freely in the surrounding suspension when viable Escherichia coli cells are killed by a heat shock. The presented results show that an amount of ions corresponding to ∼2 × 108 unit charges are released per viable bacterium killed. A micro probe station with coplanar Ti electrodes was electrically characterized and used as a measuring unit for the impedance spectroscopy recordings. This unit is compatible with common microfabrication techniques and should enable the presented method to be employed using a flow-cell device for viable bacteria counting in miniaturized on-line monitoring systems.

Place, publisher, year, edition, pages
2006. Vol. 110, no 20, 10165-10169 p.
Keyword [en]
Acoustic impedance, Cell immobilization, Electrodes, Ions, Spectroscopic analysis, Titanium, Bacteria counting, Coplanar Ti electrodes, Heat shock, Low-frequency impedance spectroscopy, Micro probe station, Bacteria
Identifiers
URN: urn:nbn:se:mdh:diva-31895DOI: 10.1021/jp060148qISI: 000237626400062Scopus ID: 2-s2.0-33745765478OAI: oai:DiVA.org:mdh-31895DiVA: diva2:936586
Available from: 2016-06-14 Created: 2016-06-14 Last updated: 2017-11-28Bibliographically approved

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