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Comparative analysis of the metagenomes extracted from wastewater treating photobioreactors
Mälardalen University, School of Business, Society and Engineering, Future Energy Center. (ACWA)ORCID iD: 0000-0002-4435-4367
University of Tartu, Estonia.ORCID iD: 0000-0001-8699-6985
University of Tartu, Estonia.ORCID iD: 0000-0003-1818-6678
Mälardalen University, School of Business, Society and Engineering, Future Energy Center.ORCID iD: 0000-0001-5480-0167
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(English)Manuscript (preprint) (Other academic)
Abstract [en]

The metagenomes of lab-scale municipal wastewater treating batch photobioreactors were studied with a focus on nitrogen metabolism, pathogen abundance and antibiotic resistance genes. Previous studies based on the dataset showed that in general, as algae growth in the reactors increased, nitrogen metabolism and virulence genes decreased. With this study, a more detailed view of these gene groups is presented.

Keyword [en]
photobioreactors, metagenomics, microalgae, wastewater treatment, pathogens, antibiotic resistance, functional analysis
National Category
Water Treatment
Research subject
Biotechnology/Chemical Engineering
Identifiers
URN: urn:nbn:se:mdh:diva-30044OAI: oai:DiVA.org:mdh-30044DiVA: diva2:885740
Funder
Knowledge Foundation, 2011006VINNOVA, 2012-01243
Available from: 2015-12-21 Created: 2015-12-21 Last updated: 2016-02-25Bibliographically approved
In thesis
1. Microbiological analysis of municipal wastewater treating photobioreactors
Open this publication in new window or tab >>Microbiological analysis of municipal wastewater treating photobioreactors
2016 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

Microalgae reactors, commonly known as photobioreactors, have become increasingly popular as an alternative for wastewater treatment. These systems reduce pollutants and remove nutrients such as nitrogen and phosphorous compounds from wastewater utilizing microalgae and bacteria. The biomass produced in the reactors can potentially be used to produce biofuels and decrease some of the energy demands of the process.

Wastewater treating photobioreactors are a relatively new technology and many aspects of their microbiology need further study. This thesis presents a broad overview of the algal and bacterial communities present in these systems by looking at the most important species, metabolic pathways and growth dynamics of both algae and bacteria.

The experiments presented in this thesis were conducted using municipal wastewater from the Västerås wastewater treatment plant. The wastewater was inoculated with algae from Lake Mälaren and compared to non-inoculated reactors. Overall, the inoculated reactors demonstrated better algal growth than those that were not inoculated. The tested systems also removed much of the ammonium and phosphorous present in the wastewater.

The dominant algae in the tested systems belonged to the genera Scenedesmus, Desmodesmus and Chlorella. In addition to algae, the systems contained a large number of bacteria, mostly from the phyla Proteobacteria and Bacteroidetes.

The algal photobioreactors contained a lower abundance of genes related to nitrogen metabolism, virulence and antibiotic resistance compared to the initial wastewater, showing that a shift in the bacterial community had occurred. The bacteria found in the systems were shown to be involved in synthesis of vitamins essential for algae growth such as vitamin B12, suggesting cooperation between the bacteria and algae.

Abstract [sv]

I takt med att världens befolkning ökar, så produceras dagligen allt mer avfall. Detta kan orsaka stora problem för miljön. När det byggs nya system för vattenrening behöver vi även ta hänsyn till kravet att minska energiåtgången. Dagens vattenreningssystem har vissa tillkortakommanden när det gäller reningsnivåer och energianvändning. Ett alternativ till dagens system, kan vara fotobioreaktorer, dvs. vattenrening med hjälp av mikroalger. Dessa system använder mikroalger och bakterier för att rena vattnet från föroreningar, kväve och fosfor.

Vattenrening med fotobioreaktorer är en relativt ny teknik. Flera aspekter gällande biologin i dessa system har ännu inte studerats i detalj. Den här avhandlingen presenterar en översikt av de alger och bakterier som är aktiva i fotobioreaktorer. Andra viktiga aspekter som tillväxt, arter samt vattenreningsförmåga har också studerats.

Ett antal försök genomfördes där alger från Mälaren tillsattes i vatten från Västerås kommunala vattenreningsanläggning. Storleken på försöken varierade mellan 250 ml och 20 liter. Det visade sig att algerna hade en bra tillväxt samt att mängden ammonium och fosfor minskade i vattnet under försöksperioden.

De alger som tillväxte mest i studien tillhörde Scenedesmus, Desmodesmus och Chlorella. Förutom alger tillväxte även ett stort antal bakterier från grupperna Proteobacteria and Bacteroidetes. Dessa bakterier visade sig syntetisera viktiga vitaminer, t.ex. vitamin B12, som algerna normalt inte kan syntetisera själva.

Sammanfattningsvis, så presenterar denna avhandling viktig information gällande alger och bakterier i en fotobioreaktor. Informationen kan vara ett viktigt bidrag till framtida utveckling av storskaliga fotobioreaktorer för vattenrening.

Place, publisher, year, edition, pages
Västerås: Mälardalen University, 2016
Series
Mälardalen University Press Dissertations, ISSN 1651-4238 ; 196
Keyword
photobioreactors, wastewater treatment, microalgae, microbiology
National Category
Water Treatment
Research subject
Energy- and Environmental Engineering
Identifiers
urn:nbn:se:mdh:diva-30045 (URN)978-91-7485-251-6 (ISBN)
Public defence
2016-01-29, Paros, Mälardalens högskola, Västerås, 09:15 (English)
Opponent
Supervisors
Funder
Knowledge Foundation, 2011006VINNOVA, 2012-01243
Available from: 2015-12-21 Created: 2015-12-21 Last updated: 2016-01-13Bibliographically approved

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Krustok, IvoOopkaup, KristjanTruu, JaakOdlare, MonicaNehrenheim, Emma

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