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Biotransformation of pink water TNT on the surface of a low-cost adsorbent pine bark
Mälardalen University, School of Business, Society and Engineering, Future Energy Center.
University of Tartu, Tartu, Estonia.
Mälardalen University, School of Business, Society and Engineering, Future Energy Center.ORCID iD: 0000-0001-5480-0167
University of Tartu, Tartu, Estonia.
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2015 (English)In: Biodegradation, ISSN 0923-9820, E-ISSN 1572-9729, Vol. 26, no 5, 375-386 p.Article in journal (Refereed) Published
Abstract [en]

This two-week anaerobic batch study evaluated 2,4,6-trinitrotoluene (TNT) removal efficiency from industrial pink water by (1) adsorption on low-cost adsorbent pine bark, and (2) adsorption coupled with TNT biotransformation by specialised microbial communities. Samples of the supernatant and acetonitrile extracts of pine bark were analysed by HPLC, while the composition of the bacterial community of the experimental batches, inocula and pine bark were profiled by high-throughput sequencing the V6 region of the bacterial 16S rRNA gene. Integrated adsorption and biotransformation proved to be the most efficient method for TNT removal from pink water. The type of applied inoculum had a profound effect on TNT removal efficiencies and microbial community structures, which were dominated by phylotypes belonging to the Enterobacteriaceae family. The analysis of acetonitrile extracts of pine bark supported the hypothesis that the microbial community indigenous to pine bark has the ability to degrade TNT.

Place, publisher, year, edition, pages
2015. Vol. 26, no 5, 375-386 p.
Keyword [en]
Microbial community structure, Next-generation sequencing, Pine bark, Pink water treatment, TNT adsorption, TNT biotransformation, Acetonitrile, Adsorption, Bioconversion, Microorganisms, Removal, RNA, Social sciences, 2, 4, 6-trinitrotoluene, High-throughput sequencing, Microbial communities, Microbial community structures, Removal efficiencies, Water treatment, Bacteria (microorganisms), Enterobacteriaceae
National Category
Earth and Related Environmental Sciences
Identifiers
URN: urn:nbn:se:mdh:diva-28715DOI: 10.1007/s10532-015-9740-7ISI: 000359264500003Scopus ID: 2-s2.0-84938961387OAI: oai:DiVA.org:mdh-28715DiVA: diva2:847883
Available from: 2015-08-21 Created: 2015-08-21 Last updated: 2015-09-07Bibliographically approved
In thesis
1. Remediation of TNT-contaminated water by using industrial low-cost residue pine bark
Open this publication in new window or tab >>Remediation of TNT-contaminated water by using industrial low-cost residue pine bark
2015 (English)Doctoral thesis, comprehensive summary (Other academic)
Abstract [en]

In the process of demilitarization of explosives, army ammunition plants generate a waste stream known as pink water. The principal component of the wastewater is the nitro-aromatic compound 2,4,6-trinitrotoluene (TNT). Although the persistence of TNT when dissolved in surface water is very limited due to its susceptibility to photo- and biotransformation, discharge of pink water to the environment has been prohibited in Sweden, the U.S. and many other countries for decades because of the toxicity of the compound and its metabolites to various ecological receptors.

The most frequently used method for treatment of pink water in Sweden today is adsorption on activated carbon, which as well as being costly, creates a sludge that must be incinerated off site.

In many countries, the timber industry residue pine bark is discarded and has no high value application. The overall aim of this thesis was to investigate the potential of pine bark for the removal of TNT from contaminated water such as pink water. Several batch studies and a column experiment were conducted. Acetonitrile extraction of pine bark and 16S rRNA sequencing for analysis of the indigenous bacterial community of pine bark were used to investigate its performance in the treatment of pink water.

The results show that pine bark has great potential as an adsorbent medium for TNT from contaminated pink water. Simultaneous use of biotransformation and adsorption methods was shown to be an improvement over adsorption alone for the removal of TNT from contaminated water bodies. Pine bark showed higher affinity towards the amino metabolites of TNT than for TNT itself. Molecular analysis of the indigenous microbial community of pine bark and chemical analysis of its acetonitrile extracts provided evidence for its ability to biotransform TNT and its metabolites. The efficiency of the transformation was enhanced by the addition of glucose and/or inoculum.

Overall, this work demonstrates the versatility of this organic industrial residue with respect to pink water treatment. Not only does it have a high affinity towards TNT and its amino metabolites, but its native microbial community even in the absence of external inoculation can also be taken advantage of, opening new possibilities for remediation of pink water.

Place, publisher, year, edition, pages
Västerås: Mälardalen University, 2015
Series
Mälardalen University Press Dissertations, ISSN 1651-4238 ; 183
National Category
Engineering and Technology
Research subject
Energy- and Environmental Engineering
Identifiers
urn:nbn:se:mdh:diva-28839 (URN)978-91-7485-226-4 (ISBN)
Public defence
2015-09-29, Gamma, Mälardalens högskola, Västerås, 10:00 (English)
Opponent
Supervisors
Funder
Knowledge Foundation
Available from: 2015-09-04 Created: 2015-09-03 Last updated: 2015-09-22Bibliographically approved

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