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A study of the antigenicity of Rickettsia helvetica proteins using two-dimensional gel electrophoresis
Mälardalen University, School of Sustainable Development of Society and Technology.
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2009 (English)In: Acta Pathologica, Microbiologica et Immunologica Scandinavica (APMIS), ISSN 0903-4641, E-ISSN 1600-0463, Vol. 117, no 4, 253-262 p.Article in journal (Refereed) Published
Abstract [en]

Hajem N, Weintraub A, Nimtz M, Romling U, Pahlson C. A study of the antigenicity of Rickettsia helvetica proteins using two-dimensional gel electrophoresis. APMIS 2009; 117: 253-62. Rickettsia helvetica is an obligate intracellular Gram-negative microorganism found in Ixodes ricinus ticks. When R. helvetica was first discovered in 1979, little was known about its physiology and it fell into oblivion until it recently was suspected of being pathogenic to humans. However, all efforts to isolate R. helvetica from patients have been unsuccessful, although serological responses against R. helvetica can be demonstrated. The aim of our study was to investigate the protein profile of R. helvetica and study the antigenicity of its proteins using two-dimensional (2D) immunoblot in order to characterize the immunological response against R. helvetica infection. Our results show that in addition to the known PS120 and OmpB antigenic R. helvetica proteins, three other antigens exist: a 60 kDa GroEL protein, a 10 kDa GroES protein and a hitherto unknown 35 kDa hypothetical protein that has similarities with ORF-RC0799 of Rickettsia conorii. Furthermore, the lipopolysaccharide showed strong antigenicity. In this study, we present the first proteome map and the first 2D immunoblot profile of R. helvetica and finally we present the 35 kDa R. helvetica as an additional antigen to the previously known rickettsial antigens.

Place, publisher, year, edition, pages
2009. Vol. 117, no 4, 253-262 p.
National Category
Natural Sciences
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URN: urn:nbn:se:mdh:diva-19901DOI: 10.1111/j.1600-0463.2009.02435.xISI: 000264608600003Scopus ID: 2-s2.0-63449120696OAI: oai:DiVA.org:mdh-19901DiVA: diva2:635697
Available from: 2013-07-05 Created: 2013-06-26 Last updated: 2013-07-05Bibliographically approved

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CiteExportLink to record
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Citation style
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