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Fluorescence in situ hybridization (FISH) for quantification of species of ammonia-oxidizing bacteria (AOB) and nitrite-oxidizing bacteria (NOB) in wastewater treatment activated sludge.
Mälardalen University, School of Sustainable Development of Society and Technology. (MERO)
Mälardalen University, School of Sustainable Development of Society and Technology. (MERO)
Mälardalen University, School of Sustainable Development of Society and Technology. (MERO)ORCID iD: 0000-0001-5480-0167
Mälardalen University, School of Sustainable Development of Society and Technology. (MERO)ORCID iD: 0000-0002-7233-6916
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2009 (English)Conference paper, Published paper (Refereed)
Place, publisher, year, edition, pages
2009.
National Category
Environmental Biotechnology
Research subject
Energy- and Environmental Engineering
Identifiers
URN: urn:nbn:se:mdh:diva-10236OAI: oai:DiVA.org:mdh-10236DiVA: diva2:347787
Conference
ASPD 5: Specialized Conference on microbial population dynamics in biological wastewater treatment (Aalborg, Denmark; 24-27 May 2009).
Available from: 2010-09-03 Created: 2010-09-03 Last updated: 2013-12-17Bibliographically approved
In thesis
1. STUDY OF BACTERIAL COMMUNITIES: – A WASTEWATER TREATMENT PERSPECTIVE
Open this publication in new window or tab >>STUDY OF BACTERIAL COMMUNITIES: – A WASTEWATER TREATMENT PERSPECTIVE
2011 (English)Licentiate thesis, comprehensive summary (Other academic)
Abstract [en]

In this thesis, the application of molecular microbiology methods to understand wastewater treatment bio-reactions is described. Two different wastewater treatment systems were chosen for the experimental work. Firstly; the activated sludge processes at two different facilities in Sweden (Västerås and Eskilstuna) were investigated and compared in a context where low temperatures can affect the efficiency of the nitrogen removal performance in terms of nitrification. Initially, fluorescence in situ hybridization (FISH) was utilised in order to quantify some of the species involved in ammonia and nitrite oxidation at Västerås, providing information on how the different communities react to decreasing temperatures. Then, the polymerase chain reaction (PCR), cloning-sequencing method was employed in order to study the composition of the ammonia oxidizing bacteria (AOB) community at the same two wastewater treatment plants (WWTPs). Secondly; the potential use of constructed wetlands for the treatment of winery wastewater was studied. High ethanol concentration artificial wastewater with and without inorganic nutrients (nitrates and phosphates) was fed in a set of pilot-scale constructed wetlands. Pollutant removal performance and enzyme activity tests were carried out. Additionally, the bacterial community structure was investigated by means of denaturing gradient gel electrophoresis (DGGE).

In the first set of studies it was shown that the AOB population which plays a major role in nitrifying reactors presented a seasonal shift and a higher diversity at Västerås during winter time, while the nitrification performance maintained stable levels and the ammonia removal efficiency increased. Thus, the higher ammonia removal efficiency at Västerås could be related to the diversity of the AOB population composition. Lastly, when constructed wetlands were in focus, the differential effects of ethanol and nutrients over the chemical oxygen demand (COD) removal performance were proven. In fact, the addition of nutrients on one of the experimental wetlands increased the COD (ethanol) removal and supported the maintenance of a bacterial population similar to the control wetland (no ethanol added). In conclusion, both studies proved a strong relationship between process performance (pollution removal) and the dynamics of the bacterial communities involved.

Place, publisher, year, edition, pages
Västerås: Mälardalen University, 2011. 57 p.
Series
Mälardalen University Press Licentiate Theses, ISSN 1651-9256 ; 129
Keyword
Activated sludge; COD removal; Constructed wetland; DGGE; FISH; Molecular microbiology; Nitrification; PCR; Wastewater treatment.
Research subject
Energy- and Environmental Engineering
Identifiers
urn:nbn:se:mdh:diva-11884 (URN)978-91-7485-007-9 (ISBN)
Presentation
2011-03-28, Gamma, Mälardalens högskola, Västerås, 10:00 (English)
Opponent
Supervisors
Available from: 2011-02-25 Created: 2011-02-25 Last updated: 2011-03-11Bibliographically approved

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CiteExportLink to record
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Citation style
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