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Genetic and phenotypic characterization of trypanosomas
Mälardalen University, School of Sustainable Development of Society and Technology.
2009 (English)Independent thesis Advanced level (degree of Master (One Year)), 20 credits / 30 HE creditsStudent thesis
Abstract [en]

 

Trypanosoma theileri, of the subgenus Megatrypanum, a non-pathogenic cosmopolitan blood dwelling parasite of bovine. T. theileri can be cultured at room temperature in several culture media.

Blood samples were collected from deer's. To see if the blood was infected with trypanosomes it was cultivated in 2 ml sheep blood or cell cultivation medium DMEM with antibiotics.

Growth was detected by microscopy to see if there were any trypanosomes.

To determine the species of trypanosomes that was in the deer blood a DNA-preparation was done before a Polymerase Chain Reaction (PCR) could be done. With sequencing the trypanosomes where determined to be Trypanosoma theileri.

Different tests were made to see in what way the trypanosomes best were caught to the objective slides.

Forty samples of borrelia positive serum from forty different patients were tested with the fluorescent microscopy. Forty different samples from blood donors were tested the same way.

Blood samples from 16 different fissiped were taken and to see if they were infected with trypanosomes. Three different PCR's were done on the 16 blood samples.

A small test on human blood was also performed.

Protein identification by immunoblot with western blot and silver staining was done.

With the electron microscopy tests were done in the ordinary way and Critical Dry Point to see if both of the techniques worked.

Enzyme-Linked Immuno Sorbent Assay (ELISA) test were accomplished on two 96 well plates. The wells on the plates were diluted in different ways before they were processed.

 

 

Place, publisher, year, edition, pages
2009. , 27 p.
Keyword [en]
trypanosoma, theileri, T.theileri, Trypanosomer, megatrypanum, PCR, cultivated, critical dry point, electron microscopy, fluorescent microscopy
National Category
Chemical Sciences Biochemistry and Molecular Biology
Identifiers
URN: urn:nbn:se:mdh:diva-6435OAI: oai:DiVA.org:mdh-6435DiVA: diva2:225302
Presentation
(English)
Uppsok
Physics, Chemistry, Mathematics
Supervisors
Examiners
Available from: 2009-10-21 Created: 2009-06-24 Last updated: 2009-10-21Bibliographically approved

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CiteExportLink to record
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Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
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Language
  • de-DE
  • en-GB
  • en-US
  • fi-FI
  • nn-NO
  • nn-NB
  • sv-SE
  • Other locale
More languages
Output format
  • html
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