The purpose of this paper is to introduce Hom-prealternative superalgebras and their bimodules. Some constructions of Hom-prealternative superalgebras and Hom-alternative superalgebras are given, and their connection with Hom-alternative superalgebras are studied. Bimodules over Hom-prealternative superalgebras are introduced, relations between bimodules over Hom-prealternative superalgebras and the bimodules of the corresponding Hom-alternative superalgebras are considered, and construction of bimodules over Hom-prealternative superalgebras by twisting is described.