The recent epidemic of the coronavirus disease showed the increased importance of controlling the transmission of contamination in the ward areas more than before. The performance of the ventilation systems in healthcare facilities can significantly impact the overall healthcare quality. This paper aims to compare two ventilation designs in an isolation room of a hospital and study the indoor airflow pattern. Computational fluid dynamics using ANSYS Fluent software was employed for the numerical simulation of the fluid flow. The simulation included the prediction of flow patterns and particle trajectories with the additional investigation into the impact of considering human thermal plume and modeling particle trajectories considering the turbulent fluctuations using the discrete random walk method in the simulation.