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This paper is focused on the blind vacant frequency channels detection in 56 MHz observation band divided into 56 channels (1 MHz each) implemented on in-house developed hardware based on the AD9364 transceiver operating in automatic gain control (AGC) mode. Vacant channel detection has been modeled as a binary hypothesis testing problem. Three signal detection algorithms widely used in the literature including energy detection, wavelets, and cyclostationary have been tested and evaluated for potential use in our target application. Primary, offline testing has been performed in the Matlab environment using the data samples captured on the target receiver's front end as a time-domain complex signal. Data samples containing one, two, or three signals generated by hardware signal generator and modulated into 2FSK, BPSK, or QPSK with symbol rate 10, 100, or 1000 kSymbol/s. The highest accuracy of 91.0 % has been observed in the offline detection for continuous wavelet transform, while energy detection has demonstrated 86.4 % accuracy. Cyclostationary detection has shown no distinguishable difference in the spectrum correlation values calculated for the AWGN noise sample and the sample containing BPSK and 2FSK modulated signals. Energy detection and discrete wavelet transform have been implemented on our target hardware and tested in the office environment in conditions that could be approximated by AWGN channel. Test sequences containing one or two signals have been generated by the signal generator and received and processed by our target radio node. Discrete wavelet transform has demonstrated 85.73 % and energy detection 85.25 % accuracy in real-time testing. © 2022 IEEE.