Electrical and optical methods for the manipulation and analyses of single cellsShow others and affiliations
2001 (English)In: CLINICAL DIAGNOSTIC SYSTEMS / [ed] Cohn, G E, Univ Washington, Dept Chem, Seattle, WA 98195 USA.: SPIE-INT SOC OPTICAL ENGINEERING , 2001, p. 1-8Conference paper, Published paper (Refereed)
Abstract [en]
This paper describes the use of focused electric fields and focused optical fields for the high resolution manipulation of single cells. A focused electric field, obtained with the use of ultramicroelectrodes (tip diameter similar to 5 mum), is used to electroporate and electrofuse individual cells selectively and with high spatial resolution. A focused optical field, in the form of an optical tweezer, is used to isolate single organelles from a cell as well as to position liposomes incorporated with receptors and transporters along the cell for the high-resolution sampling and probing of the cellular microenvironment.
Place, publisher, year, edition, pages
Univ Washington, Dept Chem, Seattle, WA 98195 USA.: SPIE-INT SOC OPTICAL ENGINEERING , 2001. p. 1-8
Series
Proceedings of SPIE, ISSN 0277-786X ; 4255
Keywords [en]
single cells, liposomes, electroporation, electrofusion, microdissection, microelectrodes, optical trapping
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:mdh:diva-51793DOI: 10.1117/12.426752ISI: 000169776800001Scopus ID: 2-s2.0-0034941454ISBN: 0-8194-3933-9 (print)OAI: oai:DiVA.org:mdh-51793DiVA, id: diva2:1478603
Conference
Conference on Clinical Diagnostic Systems, JAN 21-22, 2001, SAN JOSE, CA
2020-10-222020-10-222022-02-07Bibliographically approved