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Microfluidic device for combinatorial fusion of liposomes and cells
Göteborg University, Sweden.ORCID iD: 0000-0002-5792-7240
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2001 (English)In: Analytical Chemistry, ISSN 0003-2700, E-ISSN 1520-6882, Vol. 73, no 1, p. 126-130Article in journal (Refereed) Published
Abstract [en]

We describe an electrofusion-based technique for combinatorial synthesis of individual liposomes. A prototype device with containers for liposomes of different compositions and a fusion container was constructed. The sample containers had fluid contact with the fusion container through microchannels. Optical trapping was used to transport individual liposomes and cells through the microchannels into the fusion container. In the fusion container, selected pairs of liposomes were fused together using microelectrodes. A large number of combinatorially synthesized Liposomes with complex compositions and reaction systems can be obtained from small sets of precursor liposomes. The order of different reaction steps can be specified and defined by the fusion sequence. This device could also facilitate single cell-cell electrofusions (hybridoma production). This is exemplified by fusion of transported red blood cells.

Place, publisher, year, edition, pages
Gothenburg Univ, Dept Chem, S-41296 Gothenburg, Sweden.: AMER CHEMICAL SOC , 2001. Vol. 73, no 1, p. 126-130
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Chemical Sciences
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URN: urn:nbn:se:mdh:diva-51736DOI: 10.1021/ac000528mISI: 000166262500026PubMedID: 11195496Scopus ID: 2-s2.0-0035164186OAI: oai:DiVA.org:mdh-51736DiVA, id: diva2:1478441
Available from: 2020-10-22 Created: 2020-10-22 Last updated: 2022-03-18Bibliographically approved

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Strömberg, Anette

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