Manipulating the genetic identity and biochemical surface properties of individual cells with electric-field-induced fusionShow others and affiliations
2000 (English)In: Proceedings of the National Academy of Sciences of the United States of America, ISSN 0027-8424, E-ISSN 1091-6490, Vol. 97, no 1, p. 7-11Article in journal (Refereed) Published
Abstract [en]
A method for cell-cell and cell-liposome fusion at the single-cell level is described. Individual cells or liposomes were first selected and manipulated either by optical trapping or by adhesion to a micromanipulator-controlled ultramicroelectrode. Spatially selective fusion of the cell-cell or cell-liposome pair was achieved by the application of a highly focused electric field through a pair of 5-mu m o.d. carbon-fiber ultramicroelectrodes. The ability to fuse together single cells opens new possibilities in the manipulation of the genetic and cellular makeup of individual cells in a controlled manner, In the study of cellular networks, for example, the alteration of the biochemical identity of a selected cell can have a profound effect on the behavior of the entire network. Fusion of a single liposome with a target cell allows the introduction of the liposomal content into the cell interior as well as the addition of lipids and membrane proteins onto the cell surface. This cell-liposome fusion represents an approach to the manipulation of the cytoplasmic contents and surface properties of single cells. As an example, we have introduced a membrane protein (gamma-glutamyltransferase) reconstituted in liposomes into the cell plasma membrane.
Place, publisher, year, edition, pages
Stanford Univ, Dept Chem, Stanford, CA 94305 USA. Gothenburg Univ, Dept Chem, S-41296 Gothenburg, Sweden. Sahlgrens Univ Hosp, Inst Clin Neurosci, Dept Neurol, S-41345 Gothenburg, Sweden.: NATL ACAD SCIENCES , 2000. Vol. 97, no 1, p. 7-11
Keywords [en]
single cell, electrofusion, microelectrode, heterokaryon
National Category
Chemical Sciences
Identifiers
URN: urn:nbn:se:mdh:diva-51737DOI: 10.1073/pnas.97.1.7ISI: 000084624500004PubMedID: 10618361Scopus ID: 2-s2.0-0034602767OAI: oai:DiVA.org:mdh-51737DiVA, id: diva2:1478439
2020-10-222020-10-222022-03-18Bibliographically approved