CO2 is becoming increasingly important as a mediating fluid, and simulation studies are indispensable for corresponding developments. In this study, a simulation-based performance investigation of a solar water heating system using CO2 as a mediating fluid under sub-zero temperature condition is performed using the TRNSYS® software. The maximum performance is achieved at a solar savings fraction of 0.83 during July. The as lowest solar savingss fraction of 0.41 is obtained during December. The annual heat production of the proposed system under Fargo climate is estimated to be about 2545 kWh. An evacuated glass tube solar collector is designed, fabricated and tested for various climate conditions. Moreover, a detailed comparison of the system's performance at sub/supercritical and supercritical pressures shows that the annual heat transfer efficiency of the modeled system is 10% higher at supercritical pressure than at sub/supercritical pressures. This result can be attributd to the strong convection flow of CO2 caused by density inhomogeneities, especially in the near critical region. This condition resuls in high heat transfer rates.