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Generation of focused electric field patterns at dielectric surfaces
Chalmers University of Technology, Sweden.
Cellectricon AB, Sweden.
Cellectricon AB, Sweden. (Ytbioteknik)
University of Pittsburgh, USA.
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2005 (Swedish)In: Anal.Chem, Vol. 77, no 14, p. 4667-4672Article in journal (Other (popular science, discussion, etc.)) Published
Abstract [en]

We here report on a concept for creating well-defined electric field gradients between the boundaries of capillary electrode (a capillary of a nonconducting material equipped with an interior metal electrode) outlets, and dielectric surfaces. By keeping a capillary electrode opening close to a boundary between a conducting solution and a nonconducting medium, a high electric field can be created close to the interface by field focusing effects. By varying the inner and outer diameters of the capillary, the span of electric field strengths and the field gradient obtained can be controlled, and by varying the slit height between the capillary rim and the surface, or the applied current, the average field strength and gradient can be varied. Field focusing effects and generation of electric field patterns were analyzed using finite element method simulations. We experimentally verified the method by electroporation of a fluorescent dye (fluorescein diphosphate) into adherent, monolayered cells (PC-12 and WSS-1) and obtained a pattern of fluorescent cells corresponding to the focused electric field.

Place, publisher, year, edition, pages
2005. Vol. 77, no 14, p. 4667-4672
National Category
Materials Chemistry
Identifiers
URN: urn:nbn:se:mdh:diva-3111DOI: 10.1021/ac0502302ISI: 000230530300050Scopus ID: 2-s2.0-27544498032OAI: oai:DiVA.org:mdh-3111DiVA, id: diva2:115775
Available from: 2008-03-31 Created: 2008-03-31 Last updated: 2015-07-07Bibliographically approved

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