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A cultivation technique for E. coli fed-batch cultivations operating close to the maximum oxygen transfer capacity of the reactor
Lund Institute of Technology, Sweden.
Lund Institute of Technology, Sweden.
Mälardalen University, Department of Biology and Chemical Engineering.
Novozymes Biopharma AB, Lund, Sweden .
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2005 (English)In: Biotechnology Letters, ISSN 0141-5492, Vol. 27, no 14, 983-990 p.Article in journal (Refereed) Published
Abstract [en]

A cultivation strategy combining the advantages of temperature-limited fed-batch and probing feeding control is presented. The technique was evaluated in fed-batch cultivations with E. coli BL21(DE3) producing xylanase in a 3 liter bioreactor. A 20% increase in cell mass was achieved and the usual decrease in specific enzyme activity normally observed during the late production phase was diminished with the new technique. The method was further tested by growing E. coli W3110 in a larger bioreactor (50 l). It is a suitable cultivation technique when the O2 transfer capacity of the reactor is reached and it is desired to continue to produce the recombinant protein.

Place, publisher, year, edition, pages
2005. Vol. 27, no 14, 983-990 p.
Keyword [en]
Bioreactors/microbiology, Escherichia coli/*growth & development, Oxygen/*metabolism, Research Support; Non-U.S. Gov't
National Category
Bioprocess Technology
Identifiers
URN: urn:nbn:se:mdh:diva-2259DOI: 10.1007/s10529-005-7844-6ISI: 000231471000004PubMedID: 16132841Scopus ID: 2-s2.0-24044490195OAI: oai:DiVA.org:mdh-2259DiVA: diva2:114922
Available from: 2006-03-09 Created: 2006-03-09 Last updated: 2015-07-07Bibliographically approved

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