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Segregation to non-dividing cells in recombinant Escherichia coli fed-batch fermentation processes
Royal Institute of Technology, Sweden.
Royal Institute of Technology, Sweden.
Mälardalen University, Department of Biology and Chemical Engineering.
Mälardalen University, Department of Biology and Chemical Engineering.
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2004 (English)In: Biotechnology Letters, ISSN 0141-5492, Vol. 26, no 19, 1533-1539 p.Article in journal (Refereed) Published
Abstract [en]

In Escherichia coli fermentation processes, a drastic drop in viable cell count as measured by the number of colony forming units per ml (c.f.u. ml(-1)) is often observed. This phenomenon was investigated in a process for the production of the recombinant fusion protein, promegapoietin (PMP). After induction, the number of c.f.u. ml(-1) dropped to approximately 10% of its maximum though the biomass concentration continued to increase. Flow cytometric analysis of viability and intracellular concentration of PMP showed that almost all cells were alive and contributed to the production. Thus, the drop in the number of c.f.u. ml(-1) probably reflects a loss of cell division capability rather than cell death.

Place, publisher, year, edition, pages
2004. Vol. 26, no 19, 1533-1539 p.
Keyword [en]
Apoptosis/physiology, Bioreactors/microbiology, Cell Aggregation/physiology, Cell Survival/physiology, Colony Count; Microbial/*methods, Comparative Study, Escherichia coli/*cytology/*physiology, Fermentation/physiology, Flow Cytometry/methods, Interleukin-3, Mitosis/*physiology, Protein Engineering/*methods, Receptors; Interleukin-3/*biosynthesis/genetics, Recombinant Fusion Proteins/biosynthesis, Research Support; Non-U.S. Gov't, Thrombopoietin/*biosynthesis/genetics
National Category
Microbiology
Identifiers
URN: urn:nbn:se:mdh:diva-2044DOI: 10.1023/B:BILE.0000044458.29147.75ISI: 000225490200014PubMedID: 15604793Scopus ID: 2-s2.0-21644447627OAI: oai:DiVA.org:mdh-2044DiVA: diva2:114707
Available from: 2006-03-09 Created: 2006-03-09 Last updated: 2015-07-07Bibliographically approved

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CiteExportLink to record
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Citation style
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