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Surface-dependent conformations of human plasma fibronectin adsorbed to silica, mica, and hydrophobic surfaces, studied with use of Atomic Force Microscopy
Mälardalen University, Department of Biology and Chemical Engineering. Uppsala University, Sweden.
Uppsala University, Sweden.
Mälardalen University, Department of Biology and Chemical Engineering. Uppsala University, Sweden.
2003 (English)In: Journal of biomedical materials research. Part A., ISSN 0021-9304, Vol. 64, no 2, 349-356 p.Article in journal (Refereed) Published
Abstract [en]

Human plasma fibronectin (Fn) is a large flexible protein stabilized by intermolecular ionic interactions forming a compact structure. On altering solution conditions, the structure can revert to a more expanded state, thereby exposing previously hidden domains (e.g., cell-binding sites). Electron microscopy images of Fn air-sprayed onto mica surfaces show elongated protein structures, indicating a surface-induced structural change. This makes it interesting to investigate the influence of surface properties on the structure of adsorbed Fn. We have used intermittent-contact Atomic Force Microscopy to investigate the structure of Fn adsorbed onto mica, silica, and methylated silica surfaces. We observed that on silica surfaces, which is hydrophilic, most (70%) of the molecules had an elongated structure with partial intramolecular chain interactions, compare to molecules adsorbed on hydrophobic, methylated surfaces, where a compact structure predominated (70%). On mica surfaces, both compact and elongated protein structures were observed, with a slight preference for the elongated form (53%). Results show that surface physical properties influence the molecular structure of fibronectin on adsorption, which could provide useful information in understanding surface-induced in vivo responses.

Place, publisher, year, edition, pages
2003. Vol. 64, no 2, 349-356 p.
Keyword [en]
Absorption, Aluminum Silicates, Fibronectins/*chemistry/ultrastructure, Microscopy; Atomic Force, Models; Molecular, Protein Conformation, Research Support; Non-U.S. Gov't, Silicon Dioxide, Surface Properties
National Category
Physical Chemistry
Identifiers
URN: urn:nbn:se:mdh:diva-1840DOI: 10.1002/jbm.a.10423ISI: 000182423200018PubMedID: 12522822Scopus ID: 2-s2.0-0042008055OAI: oai:DiVA.org:mdh-1840DiVA: diva2:114503
Available from: 2006-03-09 Created: 2006-03-09 Last updated: 2015-07-06Bibliographically approved

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CiteExportLink to record
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Cite
Citation style
  • apa
  • ieee
  • modern-language-association-8th-edition
  • vancouver
  • Other style
More styles
Language
  • de-DE
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  • fi-FI
  • nn-NO
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Output format
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