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A SCANNING FORCE MICROSCOPY STUDY OF HUMAN SERUM-ALBUMIN AND PORCINE PANCREAS TRYPSIN ADSORPTION ON MICA SURFACES
Department of Chemical Engineering, Mälardalen University, Box 325, S-631 05 Eskilstuna, Sweden.
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1995 (Engelska)Ingår i: Surface Science, ISSN 0039-6028, E-ISSN 1879-2758, Vol. 325, nr 1-2, s. L406-L412Artikel i tidskrift, Letter (Refereegranskat) Published
Abstract [en]

Scanning force microscopy is more and more expanding as a tool for biological research. Here we report about the observation of molecular adsorption on mica surfaces. Human serum albumin and porcine pancreas trypsin were adsorbed on freshly cleaved mica from the solution phase, The new tapping mode (intermittent contact) scanning force microscopy technique was employed to image the molecules on the surface. We observed clusters of molecules and features which we interpret as single molecules adsorbed on the mica. For albumin, we could sometimes resolve structures corresponding to the three main domains of which albumin is composed. Analysis of lateral sizes and height, as well as scanning artefacts, are discussed. We observed the ability of the tapping mode to modify the distances between the respective domains in albumin and to split clusters into smaller parts.

Ort, förlag, år, upplaga, sidor
MALARDALEN UNIV,DEPT CHEM ENGN,S-63105 ESKILSTUNA,SWEDEN. UNIV UPPSALA,CTR BIOCHEM SEPARAT,S-75123 UPPSALA,SWEDEN.: ELSEVIER SCIENCE BV , 1995. Vol. 325, nr 1-2, s. L406-L412
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URN: urn:nbn:se:mdh:diva-66935DOI: 10.1016/0039-6028(94)00809-4ISI: A1995QH40900005Scopus ID: 2-s2.0-0029249344OAI: oai:DiVA.org:mdh-66935DiVA, id: diva2:1862344
Tillgänglig från: 2024-05-29 Skapad: 2024-05-29 Senast uppdaterad: 2024-06-19Bibliografiskt granskad

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