Healthcare is one of the sectors where gender segregation is most visible, with approximately 93% of all employees being women. Over the last decades, it has been shown that women are more frequently and longer on sick leave, as well as report higher work-related stress. This situation threatens sustainable employability in healthcare: The extent to which employees are able to work in a productive, motivated, and healthy way. In this chapter, we argue that the main reason why women in healthcare suffer increased ill health compared to men are the working conditions, that is, job demands and resources present in the environment that they share. We claim, therefore, that it is vital to study these workplace factors at a work unit level. In this chapter we discuss theoretical, methodological, and practical implications of the multilevel approach to investigating workplace factors. We outline the pattern of job demands and resources that build the working conditions in healthcare and we link them with employee productivity, health, and well-being as indicators of sustainable employability. Finally, we provide suggestions for interventions in healthcare that target distinct levels of organizational life.